Publications
- Wang, M.*, Hu, Q.*, Lv, T.*, Wang, Y.*, Lan, Q.*, Xiang, R.*, Tu, Z.*, et al., 2022. High-resolution 3D spatiotemporal transcriptomic maps of developing Drosophila embryos and larvae, Developmental Cell, 57(10), 1271-1283. *Equal contribution authors
- Koreman, G.T.*, Xu, Y.*, Hu, Q.*, Zhang Z., Allen, S.E., Wolfner, M.F., Wang, B., Han, C., 2021. Upgrading CRISPR/Cas9-mediated tissue-specific mutagenesis in Drosophila. Proceedings of the National Academy of Sciences, 118(14), e2014255118. *Equal contribution authors
- Hu, Q., Antipova, O.A., O'Halloran, T.V. and Wolfner, M.F., 2021. X-ray fluorescence microscopy scanning of Drosophila oocytes and eggs. Star Protocols, 2(1), 100247.
- Hu, Q. and Wolfner, M.F., 2020. Regulation of calcium wave initiation during Drosophila egg activation. Molecular Reproduction and Development, 87(8), 880-886.
- Hu, Q., Duncan, F.E., Nowakowski, A.B., Antipova, O.A., Woodruff, T.K., O'Halloran, T.V. and Wolfner, M.F., 2020. Zinc dynamics during Drosophila oocyte maturation and egg activation. iScience, 23(7), 101275.
- Hu, Q., Vélez-Avilés, A.N., Wolfner, M.F., 2020. Drosophila Plc21C is involved in calcium wave propagation during egg activation. Micropublication Biology. https://doi.org/10.17912/micropub.biology.000235
- York-Andersen, A.H.*, Hu, Q.*, Wood, B., Wolfner M.F., Weil, T.T., 2019. A calcium mediated actin redistribution at egg activation in Drosophila. Molecular Reproduction and Development, 87(2), 293-304. *Equal contribution authors
- Hu, Q. and Wolfner, M.F., 2019. The Drosophila Trpm channel mediates calcium influx during egg activation. Proceedings of the National Academy of Sciences, 116(38), pp.18994-19000.
Research fundings
- Resolving the spatial transcriptomic changes during Drosophila egg activation and early embryogenesis, National Natural Science Foundation of China, China, 2022-2023, host
- Determining the Role of the Conserved TRPM Ion Channel in Egg Activation, Using the Drosophila Model, National Institues of Health, United States, 2020-2022, participant
- Drosophila as a model to dissect the rise and role of calcium in egg activation, National Institutes of Health, United States, 2016-2020, participant
Postdoctoral Research (2020 – 2022)
“High resolution 3D spatiotemporal transcripomic atlases of Drosophila development”
- In collaboration with BGI-Shenzhen, used Stereo-seq to resolve the spatiotemporal transcriptomic changes during Drosophila embryonic and larval development.
3D reconstruction of a Drosophila embryo based on Stereo-seq data.
Ph.D. Thesis (2014 – 2020)
“The trigger and consequences of calcium influx during Drosophila egg activation”
Advisor: Dr. Mariana Wolfner, Cornell University
- Combined inhibitor test, germline RNAi and germline specific CRIPSR/Cas9 knockout to identify Trpm as the channel mediating calcium influx during Drosophila egg activation.
A wave of calcium sweeping through an activating fruit fly egg, Kaneuchi et al., 2015
- Visualized calcium dynamics during egg activation, engineered genetic calcium sensor and tested them using confocal and light sheet microscopy.
- Developed and optimized a highly efficient CRISPR/Cas9 mediated germline specific knockout protocol for Drosophila (collaboration with Dr. Chun Han).
- Identified region specific pressure sensitivity of mature oocytes using microneedle manipulation and microfluidic chamber (collaboration with Dr. Jan Lammerding and Dr. Chih-kuan Tung).
A calcium rise in a fruit fly oocyte induced by a microneedle
- Discovered F-actin dynamic changes and its co-regulation with calcium during Drosophila egg activation (collaboration with Dr. Timothy Weil).
X-ray fluorescence microscopy image showing the distribution of an element in fruit fly oocyte
B.S. Thesis (2013 – 2014)
“Purification and activity analysis of hydroxylase domain of JBP1, a DNA binding protein”
Advisor: Dr. Chengqi Yi, Peking University
- Constructed seven truncated protein constructs of JBP1 catalytical domain
- Attempted various methods for the purification of insoluble proteins
- Successfully purified two target protein with MBP fusion protein tag and analyzed their activity with HPLC
Summer Research Internship (2013)
“Study on the phosphorylation of TFEB by MAP4K3 in nutrient deprivation induced autophagy”
Advisor: Dr. Albert La Spada, University of California, San Diego
- Optimized several immunocytochemistry protocols for TFEB and lysosome visualization and co-localization using confocal microscopy
- Analyzed the effect of TFEB phosphorylation at serine 3 with phosphomimetic analysis
- Identified three potential proteins that may interact with MAP4K3 with co-IP assay
Presentations
- Poster: “The trigger and consequences of calcium influx during Drosophila egg activation”, Society for Developmental Biology 78th Annual Meeting, 2019
- Poster: “Dissecting the triggers of calcium wave during Drosophila egg activation”, Cornell/Pittsburgh Reproductive Genomics Symposium, 2018
- Poster: “Dissecting the triggers of calcium wave during Drosophila egg activation”, Gordon Research Conference for Fertilization and Activation of Development, 2017
- Poster: “Dissecting the mechanisms of calcium wave during Drosophila egg activation”, The Allied Genetics Conference, 2016